Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates
نویسندگان
چکیده
منابع مشابه
Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates.
Carbapenems are important agents for the therapy of infections due to multidrug-resistant Pseudomonas aeruginosa; the development of carbapenem resistance hampers effective therapeutic options. To assess the mechanisms leading to resistance, 33 clinical isolates with differing degrees of carbapenem susceptibility were analyzed for the expression of the chromosomal beta-lactamase (ampC), the por...
متن کاملInvestigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit
Background and Aims: Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections, especially in the ICU (Intensive Care Unit), which has resistance to a wide range of antibiotics, especially Carbapenems. Among the most important resistance mechanisms of this bacteria against carbapenems are MexAB-OprM efflux pump. Therefore, the aim of this study was to evaluate the g...
متن کاملInvestigation of OprD Porin Protein Levels in Carbapenem-Resistant Pseudomonas aeruginosa Isolates.
BACKGROUND The Pseudomonas aeruginosa porin OprD is a substrate-specific porin that facilitates the diffusion of basic amino acids, small peptides, and carbapenems into the cell. OprD-mediated resistance occurs as a result of decreased transcriptional expression of oprD and/or loss of function mutations that disrupt protein activity. OBJECTIVES In this study, we examined the level of oprD exp...
متن کاملinvestigation of oprd porin protein levels in carbapenem-resistant pseudomonas aeruginosa isolates
conclusions although the data support the idea that the basic mechanism of imipenem resistance could be via the loss of oprd, they do not fully explain the role of oprd and indicate that other mechanisms may play an important role. additionally, the significant decrease in the oprd levels in mdr strains suggests that oprd also plays a role in the emergence of both carbapenem and non-carbapenem ...
متن کاملCarbapenem resistance mechanisms in Pseudomonas aeruginosa clinical isolates.
In order to define the contributions of the mechanisms for carbapenem resistance in clinical strains of Pseudomonas aeruginosa, we investigated the presence of OprD, the expressions of the MexAB-OprM and MexEF-OprN systems, and the production of the beta-lactamases for 44 clinical strains. All of the carbapenem-resistant isolates showed the loss of or decreased levels of OprD. Three strains ove...
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ژورنال
عنوان ژورنال: The Journal of Infection in Developing Countries
سال: 2018
ISSN: 1972-2680
DOI: 10.3855/jidc.9486